The intracellular positioning of the nucleus has gained substantial interest among biologists due its relevance in cell cycle, differentiation, migration, and polarity. Abnormal positioning has been related to cell and tissue function deficiency and severe defects in embryogenesis. Membrane linkers and cytoskeletal and molecular motor dynamics are essential factors for nuclear movement. However, understanding the coordination and the quantification of force generating elements for nuclear positioning are current challenges. In this project, we investigate time-resolved nuclear distribution starting at the earliest stage of Drosophila embryo development to understand causality and regulation of nuclear positioning. We infer on how predefined external factors (e.g. neighbor nuclei, cytoplasmic volume, geometry) influence the dynamics of the cytoskeletal machinery surrounding a nucleus, with focus on microtubules, actin, associated molecular motors and linking proteins.