Neurobiologia Molecular

Diogo Castro

O objetivo principal do grupo de investigação é compreender como são regulados os programas de expressão genética global durante a neurogénese de vertebrados.

Os investigadores utilizam modelos de células estaminais cancerígenas de Glioblastoma Multiforme, o tumor cerebral mais frequente e agressivo. Através de uma abordagem multidisciplinar, os investigadores combinam técnicas de genómica e biologia de células estaminais.

Projetos

NotchBookmark – Estudo da função de marcador mitótico de RBPJ/Notch na neurogénese de vertebrados.

Ficha de projeto

Publicações

Vasconcelos, F.F., Sessa, A., Laranjeira, C., Raposo, A.A.S.F., Teixeira, V., Hagey, D.W., Tomaz, D.M., Muhr, J., Broccoli, V., Castro, D.S. (2016) MyT1 counteracts the neural progenitor program to promote vertebrate neurogenesis Cell Rep. 17(2) : 469–483
Singh, S., Howell, D., Trivedi, N., Kessler, K., Ong, T., Rosmaninho, P., Raposo, A.A.S.F., Robinson, G., Roussel, M.F., Castro, D.S.* , Solecki, D.J.* *Shared corresponding author (2016) Zeb1 controls neuron differentiation and germinal zone exit by a mesenchymal-epithelial-like transition. eLife 5 : e12717

Raposo, A.A.S.F., Vasconcelos, F.F., Drechsel, D., Marie, C., Johnston, C., Dolle, D., Bithell, A., Guillotin, S., van den Berg, D.L.C., Etwiller, L., Flicek, P., Crawford, G.E., Parras, C.M, Berninger, B., Buckley, N.J., Guillemot, F., Castro, D.S. (2015) Ascl1 coordinately regulates gene expression and the chromatin landscape during neurogenesis Cell Rep pii : S2211-1247(15)00171-0
Mateo, J.L., van den Berg, D.L., Haeussler, M., Drechsel, D., Gaber, Z.B., Castro, D.S. , Robson, P., Crawford, G.E., Flicek, P., Ettwiller, L., Wittbrodt, J., Guillemot, F., Martynoga, B. (2015) Characterization of the neural stem cell gene regulatory network identifies OLIG2 as a multifunctional regulator of self-renewal Genome Res 25(1) : 41-56
Vasconcelos, F.F., Castro, D.S. (2014) Transcriptional control of vertebrate neurogenesis by the proneural factor Ascl1 Front Cell Neurosci 8 : 412
Borromeo, M.D., Meredith, D.M., Castro, D.S. , Chang, J.C., Tung, K.C., Guillemot, F., Johnson, J.E. (2014) A transcription factor network specifying inhibitory versus excitatory neurons in the dorsal spinal cord Development 141 : 2803-2812
Wapinski, O.L., Vierbuchen, T., Qu, K., Lee, Q.Y., Chanda, S., Fuentes, D.R., Giresi, P.G., Ng YH, Marro, S., Neff, N.F., Drechsel, D., Martynoga, B., Castro, D.S. , Webb, A.E., Südhof TC, Brunet, A., Guillemot, F., Chang, H.Y., Wernig, M. (2013) Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons Cell 155(3) : 621-35
Castro, D.S. , Guillemot, F. (2011) Old and new functions of proneural factors revealed by the genome-wide characterization of their transcriptional targets Cell Cycle 10(23) : 4026-4031
Castro, D.S.* , Martynoga, B., Parras, C., Ramesh, V., Pacary, E., Johnston, C., Drechsel, D., Lebel-Potter, M., Galinanes-Garcia, L., Hunt, C., Dolle, D., Bithell, A., Ettwiller L, Buckley, N., Guillemot, F. *Corresponding author (2011) A novel function of the proneural factor Ascl1 in progenitor proliferation identified by genome-wide characterization of its targets Genes & Dev 25 : 930-945