Protein- Nucleic Acids Interactions
Sequence is not fate. Proteins demonstrate an impressive ability not only to recognize and bind to particular pieces of nucleic acids code but also to alter its information content by catalysing reactions that rearrange its sequence or specifically change one nucleotide for another. Organisms have found in such mechanisms the means for creating sequence diversity as it is gloriously exemplified in the diversification of immunoglobulin genes. The recent realization that multi-cellular organisms achieve phenotypic complexity without a parallel increase in number of genes has highlighted the importance of posttranscriptional RNA modifications in creating and fine-tuning a much larger repertoire of proteins originating from a small number of genes. I am interested in the study of the molecular mechanisms involved in such diversification of RNA and DNA sequence as well as understanding the consequences of such processes for molecular evolution dynamics. In this direction I am employing the tools of computational, molecular and structural biology in the study of RNA and DNA editing. My work presently focuses on the A to I RNA editing process which alters the sequence of thousands of human pre-mRNAs (Athanasiadis et al., 2004), having this way a prominent role in regulating Innate Immune responses to dsRNA.
You can express your interest by sending your CV to Alekos Athanasiadis.
- Molecular basis of the recognition of foreign nucleic acids in innate immunity
- Computational modeling of the A to I RNA editing process.
- Rakus, K., Ronsmans, M., Forlenza, M., Boutier, M., Piazzon, M.C., Jazowiecka-Rakus, J., Gatherer, D., Athanasiadis, A., Farnir, F., Davison, A.J., P. Boudinot, P., Michiels, T., Wiegertjes, G.F., Vanderplasschen, A. (2017) Conserved fever pathways across vertebrates: A Herpesvirus expressed decoy TNF-alpha receptor delays behavioral fever in fish. Cell Host Microbe 21(2) : 244-253
- Kus, K., Rakus, K., Boutier, M., Tsigkri, T., Gabriel, L., Vanderplasschen, A., Athanasiadis, A. (2015) The Structure of the Cyprinid herpesvirus 3 ORF112-Zalpha.Z-DNA Complex Reveals a Mechanism of Nucleic Acids Recognition Conserved with E3L, a Poxvirus Inhibitor of Interferon Response. J Biol Chem 290 : 30713-30725
- Koyama, T., Rodrigues, M. A., Athanasiadis, A., Shingleton, A. W., Mirth, C. K. (2014) Nutritional control of body size through FoxO-Ultraspiracle mediated ecdysone biosynthesis. eLife 3:e03091
- Remy, E., Cabrito, T.R., Batista, R.A., Hussein, M.A., Teixeira, M.C., Athanasiadis, A., Sa-Correia, I., Duque, P. (2014) Intron retention in the 5'UTR of the novel ZIF2 transporter enhances translation to promote zinc tolerance in Arabidopsis PLoS Genet 10 : e1004375
- de Rosa, M., Zacarias, S., Athanasiadis, A. (2013) Structural basis for Z-DNA binding and stabilization by the zebrafish Z-DNA dependent protein kinase PKZ Nucleic Acids Res 41 : 9924-9933
- Athanasiadis, A. (2012) Zalpha-domains: at the intersection between RNA editing and innate immunity Seminars in Cell & Developmental Biology 23 : 275-280
- Santos, M.E., Athanasiadis, A., Leitao, A.B., Dupasquier, L., Sucena, E. (2011) Alternative splicing and gene duplication in the evolution of the FoxP gene sub-family Mol Biol Evol 28 : 237-247
- de Rosa, M., de Sanctis, D., Rosario, A.L., Archer, M., Rich, A., Athanasiadis, A.*, Carrondo, M.A. * Corresponding Author (2010) Crystal structure of a junction between two Z-DNA helices Proc Natl Acad Sci U S A 107 : 9088-9092
- Placido, D., Brown, B.A., 2nd, Lowenhaupt, K., Rich, A., Athanasiadis, A. (2007) A left-handed RNA double helix bound by the Z alpha domain of the RNA-editing enzyme ADAR1 Structure 15 : 395-404
- Athanasiadis, A., Rich, A., Maas, S. (2004) Widespread A-to-I RNA editing of Alu-containing mRNAs in the human transcriptome PLoS Biol 2 : e391
Alekos Athanasiadis, Principal Investigator
PhD in Structural Biology, University of Crete/IMBB, Greece
Phone +351 214 464 648
E-mail: [email protected]