Physical principles of nuclear division
The research group is a multidisciplinary team interested in the physical aspects of intracellular organization.
As a model system, they study the earliest stage of Drosophila development, from oogenesis to the mature egg to fertilization and blastoderm cleavages. On one hand, they focus on pronuclear fusion in the fertilized egg and how the syncytial embryo defines the inter-nuclear distance between rapid mitotic divisions. On the other hand, researchers study the physical and biochemical rules determining oocyte polarity.
They use a variety of approaches from chemistry, molecular biology, genetic engineering, micromechanics and optical microscopy.
Their core experimental platform is a cytoplasmic explant assay using single oocytes, mature eggs or early embryos.
- Physical principles of nuclear migration and position in Drosophila syncytial embryos
- Cytoskeletal dynamics during inter-nuclear spacing in the Drosophila syncytial embryo
- Reconstitution of insect fertilization to study intracellular bacteria transmission
- Reconstitution of cell polarity and axis determination in a cell-free system
- Silva A.M., Osório D.S., Pereira A.J., Maiato H., Pinto I.M., Rubinstein B., Gassmann R., Telley I.A., Carvalho A.X. (2016) Robust gap repair in the contractile ring ensures timely completion of cytokinesis. J Cell Biol. 215(6) : 789-799
- Telley I.A., Gaspar I., Ephrussi A., Surrey T. (2013) A single Drosophila embryo extract for the study of mitosis ex vivo. Nat Protoc. 8(2) : 310-324
- Telley I.A., Gaspar I., Ephrussi A., Surrey T. (2012) Aster migration determines the length scale of nuclear separation in the Drosophila syncytial embryo. J Cell Biol. 197 : 887-895
- Telley I.A., Bieling P., Surrey T. (2011) Reconstitution and quantification of dynamic microtubule end tracking in vitro using TIRF microscopy. Methods Mol Biol. 777 : 127-45
- Bieling P.*, Telley I.A.*, Hentrich C., Piehler J., Surrey T. (2010) Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor and +TIP dynamics. Methods Cell Biol. 95 : 549-574
- Bieling P., Telley I.A., Surrey T. (2010) A minimal midzone protein module controls formation and length of antiparallel microtubule overlaps. Cell 142 : 420-432
- Telley I.A., Bieling P., Surrey T. (2009) Obstacles on the microtubule reduce the processivity of Kinesin-1 in a minimal in vitro system and in cell extract. Biophys J. 96 : 3341-3353
- Sung H., Telley I.A., Papadaki P., Ephrussi A., Surrey T., Rørth P. (2008) DrosophilaEnsconsin promotes productive recruitment of Kinesin-1 to microtubules. Dev Cell. 15 : 866-876
- Bieling P., Kandels-Lewis S., Telley I.A., van Dijk J., Janke C., Surrey T. (2007) CLIP-170 tracks groing microtubule ends by dynamically recognizing composite EB1/tubulin binding sites. J Cell Biol. 183 : 1223-1233
- Telley I.A., Denoth J., Stüssi E., Pfitzer G., Stehle R. (2006) Half-sarcomere dynamics in myofibrils during activation and relaxation studied by tracking fluorescent markers. Biophys J. 90(2) : 514-530
- 17 jun 2020
Ivo Telley, Principal Investigator
PhD in Mechanical Engineering, ETH, Zurich, Switzerland
Phone: +351 214464606
E-mail: [email protected]