One third of proteins encoded by eukaryotic genomes undergo their biogenesis in an essential membranous organelle called the endoplasmic reticulum (ER)—the cell’s protein factory. Many of these proteins are integral membrane proteins, which must fold in the ER and be inserted into the ER membrane. Following their successful membrane import, these proteins are trafficked through a network of membranous organelles called the secretory pathway.
When membrane proteins are trafficked to their final destination (e.g. the plasma membrane) they fulfill multiple important physiological roles essential for eukaryotic life, including cell signaling.
Surprisingly, the complex biogenesis of many signaling proteins, including those associated with human diseases, remains poorly understood. This, combined with the fact that approximately 60% of current drugs target membrane proteins, illustrates the importance to understand how membrane proteins are successfully made, integrated into the membrane and trafficked within the secretory pathway.
The Membrane Traffic lab is interested in several aspects of membrane protein homeostasis (‘Proteostasis’) including how membrane proteins are made, degraded or trafficked within the secretory pathway.
We use a combination of cellular biochemistry and mouse physiology in our experimental approaches, focusing particularly on models of inflammation and/or metabolic homeostasis.
Our work currently focuses on three areas being developed:
- How membrane trafficking regulates signalling controlled by the metalloprotease ADAM17/TACE.
- Role of quality control in the secretory pathway in vivo in mice, during development and disease.
- Genetic screens to identify novel trafficking factors.
- Cavadas, M., Oikonomidi, I., Gaspar, C.J., Burbridge, E., Badenes, M., Félix, I., Bolado, A., Hu, T., Bileck, A., Gerner, C., Domingos, P.M., von Kreigsheim, A., Adrain C. (2017) Phosphorylation of iRhom2 Controls Stimulated Proteolytic Shedding by the Metalloprotease ADAM17/TACE. Cell Rep. 21 : 745–757
- Johnson, N., Březinová, J., Stephens, E., Burbridge, E., Freeman, M., Adrain, C*., Strisovsky*, K. (2017) Quantitative proteomics screen identifies a substrate repertoire of rhomboid protease RHBDL2 in human cells and implicates it in epithelial homeostasis. Sci Rep. 7 : 4277
- Adrain, C., Zettl, M., Christova, Y., Taylor, N., Freeman, M. (2012) Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. (joint-first author). Science 355 : 225-8
- Zettl, M., Adrain, C. , Strisovsky, K., Lastun, V., Freeman, M. (2011) Rhomboid family pseudoproteases use the ER quality control machinery to regulate intercellular signaling. Cell 145 : 79-91
- Adrain, C., Strisovsky, K., Zettl, M., Hu, L., Lemberg, M.K., Freeman, M. (2011) Mammalian EGF receptor activation by the rhomboid protease RHBDL2. EMBO Rep. 12 : 421-7
- Adrain, C. , Duriez, P.J., Brumatti, G., Delivani, P., Martin, S.J. (2006) The cytotoxic lymphocyte protease, granzyme B, targets the cytoskeleton and perturbs microtubule polymerization dynamics J Biol Chem 281 : 8118-25
- Delivani, P., Adrain, C. , Taylor, R.C., Duriez, P.J., Martin, S.J. (2006) Role for CED-9 and Egl-1 as regulators of mitochondrial fission and fusion dynamics Mol Cell 21 : 761-73
- Adrain, C. , Murphy, B.M., Martin, S.J. (2005) Molecular ordering of the caspase activation cascade initiated by the CTL/NK protease, Granzyme B J. Biol. Chem 280 : 4663-4673
- Adrain, C. , Creagh, E.M., Cullen, S.P., Martin, S.J. (2004) Caspase-dependent inactivation of proteasome function during programmed cell death in Drosophila and man (joint-first author) J. Biol. Chem 279 : 36923-36930
- Adrain, C., Creagh, E.M., Martin, S.J. (2001) Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2.EMBO J. 20 : 6627-36
Colin Adrain, Principal Investigator
PhD in Molecular & Cell Biology Trinity College, Dublin, Ireland
Phone: +351 214 407 904
Email: [email protected]