The area of research interests of the group led by Isabel Gordo is Evolutionary Biology, with a great focus on microbial evolution. The research group combine both theoretical and empirical work with the aim at a better understanding of the major forces that shape variation in bacterial populations.
Present and future projects of the research team include:
- Study the process of adaptation in the context of ecosystems using Escherichia coli as a model organism.
- Test theoretical models of adaptive evolution against genotypic and phenotypic data obtained in experimentally adapted bacterial populations.
- Determine the level of epistatic interactions on fitness between mutations that confer resistance to commonly used antibiotics.
- Study the evolution of mutation rates and determine the factors that influence polymorphism for mutation rates in bacterial populations.
Epistasis and antibiotic resistance
Even with the success of antibiotics, the rapid evolution of multiple antibiotic resistances constitutes an increasing health problem. We are studying the importance of genetic interactions in the evolution of antibiotic resistance. In particular the costs of multiple resistance and the rate of comp
Bacteria evolution in the mammalian gut
In this project we aim at determine the speed and mode of adaptation of Escherichia coli close to its natural environments. We used different strains of mice and a commensal strain of E. coli to unravel the dynamics of adaptation in this complex ecosystem. We are interesting in modeling the process
- Frazão, N., Sousa, A., Lässig, M., Gordo, I. (2019) Horizontal gene transfer overrides mutation in Escherichia coli colonizing the mammalian gut. PNAS.
- Sousa, A., Ramiro, R.S., Barroso-Batista, J., Güleresi, D., Lourenço, M., Gordo, I.(2017) Recurrent reverse evolution maintains polymorphism after strong bottlenecks in commensal gut bacteria. Mol Biol Evol. msx221
- Sousa, A., Frazão, N., Ramiro, R.S., Gordo, I. (2017) Evolution of commensal bacteria in the intestinal tract of mice. Curr Opin Microbiol. 38 : 114-121
- Moura de Sousa, J., Balbontín, R., Durão, P. , Gordo, I. (2017) Multidrug-resistant bacteria compensate for the epistasis between resistances PLoS Biol. 15 (4) : e2001741
- Durão, P., Güleresi, D., Proença, J., Gordo, I. (2016) Enhanced survival of Rifampicin and Streptomycin double resistant E. coli inside macrophages.Antimicrob Agents Chemother. 60(7) : 4324-32
- Barroso-Batista, J., Demengeot, J., Gordo, I. (2015) Adaptive immunity increases the pace and predictability of evolutionary change in commensal gut bacteria Nat Commun. 6 : 8945
- Barroso-Batista, J., Sousa, A., Lourenço, M., Bergman, M-L., Sobral, D., Demengeot, J., Xavier, K.B., Gordo, I. (2014) The first steps of adaptation of Escherichia coli to the gut are dominated by soft sweeps. PLoS Genet. PLoS Genet. 10(3) : e1004182
- Miskinyte, M., Sousa, A., Ramiro, R.S., de Sousa, J.A.M., Kotlinowski, J., Caramalho, I., Magalhães, S., Soares, M.P., Gordo, I. (2013) The genetic basis of Escherichia colipathoadaptation to macrophages. PLoS Pathog. 9(12) : e1003802
- Sousa, A., Magalhães, A., Gordo, I. (2012) Cost of antibiotic resistance and the geometry of adaptation. Mol Biol Evol. 29(5) : 1417-28
- Trindade, S., Sousa, A., Xavier, K.B., Dionisio, F., Ferreira, M.G., Gordo, I. (2009) Positive Epistasis Drives the Acquisition of Multidrug Resistance PLoS Genet. 5(7) : e1000578
- Perfeito, L., Fernandes, L., Mota, C., Gordo, I. (2007) Adaptive Mutations in Bacteria: High Rate and Small Effects. Science. 317(5839) : 813-5
Isabel Gordo, Principal Investigator
PhD in Evolutionary Biology, University of Edinburgh, United Kingdom
Phone: +351 214407915
E-mail: [email protected]