Cell Biology of Viral Infection
Maria João Amorim
Influenza A virus is one of the major causes of acute contagious respiratory disease in humans, leading to seasonal epidemics and sporadic deadly pandemics.
Despite tight surveillance of circulating strains worldwide, and the implementation of yearly vaccination schemes, the pathogen is responsible for high mortality, morbidity and economic damage. Development of novel ways to control infection is therefore necessary. The elucidation of host factors contributing to a productive infection, as well as host pathways that are able to regulate it can lead to novel therapies.
The Cell Biology of Viral Infection Lab is interested in understanding the interactions between influenza A virus and the infected host at different levels. By identifying the host machinery necessary to sustain viral infection we seek to understand the viral lifecycle and unravel key aspects of the biology of the cell.
Our goal is to explain if the viral usage of a certain pathway induces alterations to its normal functioning and to the overall morphology of the cell. By studying the host response to viral challenge, we seek to unravel host innate immune responses and ways to control viral infection.
Mechanisms of virus assembly
Viral assembly is a fascinating molecular biology process. Starts once a virus enters a cell and replicates.
Influenza A virus virulence factors
Influenza A virus constitutes a serious health problem being responsible for a quarter to half a million deaths annually.
Ho, J S Y, Angel, M, Ma, Y, Sloan, E, Wang, G, Martinez-Romero, C, Alenquer, M, Roudko, V, Chung, L, Zheng, S, Chang, M, Fstkchyan, Y, Clohisey, S, Dinan, A M, Gibbs, J, Gifford, R, Shen, R, Gu, Q, Irigoyen, N, Campisi, L, Huang, C, Zhao, N, Jones, J D, van Knippenberg, I, Zhu, Z, Moshkina, N, Meyer, L, Noel, J, Peralta, Z, Rezelj, V, Kaake, R, Rosenberg, B, Wang, B, Wei, J, Paessler, S, Wise, H M, Johnson, J, Vannini, A, Amorim, M J, Baillie, J K, Miraldi, E R, Benner, C, Brierley, I, Digard, P, Łuksza, M, Firth, A E, Krogan, N, Greenbaum, B D, MacLeod, M K, van Bakel, H, Garcìa-Sastre, A, Yewdell, J W, Hutchinson, E, Marazzi, I, Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection, 2020, Cell.
- Alenquer M, Vale-Costa S, Sousa AL, Etibor TA, Ferreira F and Amorim MJ, Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites, 2019, Nature Communications. 10, Article number: 1629. Top 2% in Biochemistry, Genetics and Molecular Biology.
Also see Comment
- Nieto A, Vasilijevic J, Santos NB, Zamarreño N, Amorim MJ, Falcon A, Mutation S110L of H1N1 influenza virus hemagglutinin: A potent determinant of attenuation in the mouse model, 2019, 10, Article 132. Top 12% in Immunology.
- Amorim MJ, A comprehensive review on the interaction between the host GTPase Rab11 and influenza A virus, 2019, Frontiers in Cell and Developmental Biology, 6 Article 176. Top 13% in Cell Biology.
- Pereira CF, Wise HM, Kurian D, Pinto RM, Amorim MJ, Gill AC, Digard P, Pleotropic effects of mutations in the effector domain of influenza A virus NS1 protein, BMC Research Notes, 2018, BMC Research notes, 11:673.
- Ramos-Nascimento A, Kellen B, Ferreira F, Alenquer M, Vale-Costa S, , Raposo G, Delevoye C and Amorim MJ, KIF13A mediates influenza A virus ribonucleoproteins trafficking, Journal of Cell Science, 2017, 130:4038-4050. Top 12% in Cell Biology.
- Pereira CF, Read EKC, Wise HM, Amorim MJ and Digard P, The influenza A virus NS1 protein promotes efficient nuclear export of unspliced viral M1 mRNA, 2017, J. Virology, 91, pii: e00528-17.
- Sousa AL, Vale-Costa S, Amorim MJ and Tranfield EM, Using correlative light and electron microscopy to understand influenza A viral assembly, Ultrastructural Pathology, 2017, 41:80-81.
- Vale-Costa S, Alenquer M, Sousa AL, Kellen B, Ramalho J, Tranfield EM and Amorim MJ, Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors, Journal of Cell Science, 2016, 129: 1697-1710.
- Vale-Costa S and Amorim MJ, Clustering Rab11 vesicles in influenza A virus infected cells creates hotspots containing the 8 viral ribonucleoproteins, Small GTPases, 2016, 23: 0-7.
- Vale-Costa S and Amorim MJ, Recycling endosomes and Viral Infection, Viruses, 2016, 8: 64-92.
- Alenquer M, and Amorim MJ, Exosomes in viral infection: More than ESCRT(ing) viral budding, Viruses, 2015, 7: 5066-5083.
- Amorim MJ*, Kao R and Digard P, Nucleozin Targets Cytoplasmic Trafficking of Viral Ribonucleoprotein-Rab11 Complexes in Influenza A Virus Infection, J. Virology, 2013, 87(8):4694-4703 (Research article).
Maria João Amorim, Principal Investigator
PhD in Virology University of Cambridge, United Kingdom
Phone: +351 214 407 905
E-mail: [email protected]