Transgenics: Mouse

The Mouse Transgenics Unit is a core facility that provides transgenic and gene targeting mice to investigators of IGC, as well as to investigators within Portugal and abroad. For externals is fee-for-service based. We are competitive in success rates and pricing.
The Unit performs microinjections of DNA into fertilized embryos to generate transgenic mice, microinjections of gene-targeted ES cells into host blastocysts to generate gene knock-out or knock-in mice and of CRISPR/Cas9 and gRNAs as well as DNA donors into mouse zygotes for gene editing projects, using the ultimate technologies in the field. We provide users general consultations.

Location: Mallo Lab
Contacts: + 351 214 464 525 @: [email protected]

 

The researchers provide the DNA constructs, gRNAs or ES cells. The TU staff then performs all microinjections and surgical procedures required for the production of the mutant mice. We deliver founders or chimeras that are then further crossed and analyzed by the requesting researchers.


EQUIPMENT

Leica DMI4000B automated inverted research microscope
NEPA21 electroporator
FemtoJet, Eppendorf

SERVICES

The Transgenics Unit is dedicated to producing genetically modified mice via transgenic and gene targeting methodologies, using state of the art techniques.


USEFUL RESOURCES


PEOPLE


MEDIA


Consortiums

         

Updated on 28 april 2022

Cookies settings

Cookies Selection

The Calouste Gulbenkian Foundation uses cookies to improve your browsing experience, security, and its website performance. The Foundation may also use cookies to share information on social media and to display messages and advertisements personalised to your interests, both on our website and in others.