Plant Molecular Biology
As sessile organisms, plants have evolved unique strategies to cope with environmental challenges that affect their growth and development. These range from morphological and physiological changes to alterations at the cellular level, but the basis for adaptation or acclimation lies ultimately at the level of the genome.
The Plant Molecular Biology group uses Arabidopsis thaliana as a model system to investigate how plants perceive and respond to environmental stress at the molecular level. In particular, we are focusing on the role of RNA alternative splicing in the regulation of gene expression. The versatility of this posttranscriptional regulatory mechanism suggests an important contribution in ensuring the developmental plasticity and stress tolerance essential for plant survival.
Another major project in the lab is uncovering a role for membrane transporters of the Major Facilitator Superfamily (MFS) in plant development and responses to abiotic stress. Interestingly, the functional analysis of these membrane proteins is revealing striking examples of the biological impact of alternative splicing in plants.
- Functional significance of alternative splicing and SR proteins in plant responses to abiotic stress
- Roles of MFS membrane transporters in plant development and abiotic stress tolerance
- Niño-González M1, Novo-Uzal E1, Richardson DN, Barros PM, Duque P* (2019) More transporters, more substrates: The arabidopsis Major Facilitator Superfamily revisited. Molecular Plant 12(9): 1182-1202.
- Laloum T, Martín G, Duque P* (2018) Alternative splicing control of abiotic stress responses. Trends in Plant Science 23(2): 140-150.
- Remy E, Niño-González M, Godinho CP, Cabrito, TR, Teixeira, MC, Sá-Correia I, Duque P* (2017) Heterologous expression of the yeast Tpo1p or Pdr5p membrane transporters in Arabidopsis confers plant xenobiotic tolerance. Scientific Reports 7: 4529
- Remy E, Duque P* (2016) Assessing tolerance to heavy-metal stress in Arabidopsis thaliana seedlings. Methods in Molecular Biology 1398: 197-208
- Carvalho RF, Szakonyi, D, Simpson CG, Barbosa ICR, Brown JWS., Baena-González E, Duque P* (2016) The Arabidopsis SR45 splicing factor, a negative regulator of sugar signaling, modulates SNF1-related protein kinase 1 (SnRK1) stability. The Plant Cell 28(8) : 1910-25
- Remy E, Cabrito TR, Batista RA, Teixeira MC, Sá-Correia I, Duque P* (2015) The Major Facilitator Superfamily transporter ZIFL2 modulates cesium and potassium homeostasis in Arabidopsis. Plant & Cell Physiology 56(1) : 148-162
- Remy E, Cabrito TR, Batista RA, Hussein MAM, Teixeira MC, Athanasiadis A, Sá-Correia I, Duque P* (2014) Intron retention in the 5’UTR of the novel ZIF2 transporter enhances translation to promote zinc tolerance in Arabidopsis. PLoS Genetics 10(5) : e1004375
- Remy E, Cabrito TR, Baster P, Batista RA, Teixeira MC, Friml J, Sá-Correia I, Duque P* (2013) A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. The Plant Cell 25(3) : 901-926
- Carvalho SD, Saraiva R, Maia TM, Abreu IA, Duque P* (2012) XBAT35, a novel Arabidopsis RING E3 ligase exhibiting dual targeting of its splice isoforms, is involved in ethylene-mediated regulation of apical hook curvature. Molecular Plant 5(6) : 1295-1309
- Remy E, Cabrito TR, Batista RA, Teixeira MC, Sá-Correia I, Duque P* (2012) The Pht1;9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation. New Phytologist 195(2) : 356-371
- Carvalho RF, Carvalho SD, Duque P* (2010) The plant-specific SR45 protein negatively regulates glucose and ABA signaling during early seedling development in Arabidopsis. Plant Physiology 154(2) : 772-783
Paula Duque, Principal Investigator
PhD in Applied Plant Biology, Faculdade de Ciências, Universidade de Lisboa, Portugal
Phone: +351 214464630
E-mail: [email protected]